Cautionary notes on the use of NF-κB p65 and p50 antibodies for CNS studies

BACKGROUND: The characterization and cellular localization of transcription factors like NF-κB requires the use of antibodies for western blots and immunohistochemistry. However, if target protein levels are low and the antibodies not well characterized, false positive data can result. In studies of...

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Bibliographic Details
Main Authors: Herkenham, Miles, Rathore, Priyanka, Brown, Pierre, Listwak, Samuel J
Format: Artigo
Language:Inglês
Published: BioMed Central 2011
Subjects:
Research
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC3210105/
https://ncbi.nlm.nih.gov/pubmed/21999414
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1742-2094-8-141
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