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Cautionary notes on the use of NF-κB p65 and p50 antibodies for CNS studies

BACKGROUND: The characterization and cellular localization of transcription factors like NF-κB requires the use of antibodies for western blots and immunohistochemistry. However, if target protein levels are low and the antibodies not well characterized, false positive data can result. In studies of...

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Autors principals: Herkenham, Miles, Rathore, Priyanka, Brown, Pierre, Listwak, Samuel J
Format: Artigo
Idioma:Inglês
Publicat: BioMed Central 2011
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC3210105/
https://ncbi.nlm.nih.gov/pubmed/21999414
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1742-2094-8-141
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