A simple and efficient method for the separation and detection of small DNA fragments by electrophoresis in formamide containing agarose gels and Southern blotting to DBM-paper.

We show in this report that DNA fragments smaller than 300 bp are separated with high resolution by electrophoresis in concentrated (up to 7%) agarose gels containing 50% formamide. The separated DNA fragments can subsequently be quantitatively transferred to DBM-paper [Alwine, J.C. et al., Proc. Na...

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Detalhes bibliográficos

Publicado no:	Nucleic Acids Res
Principais autores:	Sun, Y L, Xu, Y Z, Chambon, P
Formato:	Artigo
Idioma:	Inglês
Publicado em:	Oxford University Press 1982
Assuntos:	Research Article
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC320927/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/7145714/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/10.19.5753
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