A simple and efficient method for the separation and detection of small DNA fragments by electrophoresis in formamide containing agarose gels and Southern blotting to DBM-paper.
We show in this report that DNA fragments smaller than 300 bp are separated with high resolution by electrophoresis in concentrated (up to 7%) agarose gels containing 50% formamide. The separated DNA fragments can subsequently be quantitatively transferred to DBM-paper [Alwine, J.C. et al., Proc. Na...
Gardado en:
| Publicado en: | Nucleic Acids Res |
|---|---|
| Principais autores: | , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado: |
Oxford University Press
1982
|
| Assuntos: | |
| Acceso en liña: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC320927/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/7145714/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/10.19.5753 |
| Tags: |
Sen Etiquetas, Sexa o primeiro en etiquetar este rexistro!
|