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Determination of Borrelia Surface Lipoprotein Anchor Topology by Surface Proteolysis

We used a surface trypsinolysis assay to probe accessibility of the membrane-proximal N-terminal tether peptides of Borrelia surface lipoproteins OspA and Vsp1. Our findings with both wild-type and mutant proteins are only compatible with the anchoring of these surface lipoproteins in the outer leaf...

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Autori principali: Chen, Shiyong, Kumru, Ozan S., Zückert, Wolfram R.
Natura: Artigo
Lingua:Inglês
Pubblicazione: American Society for Microbiology 2011
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC3209212/
https://ncbi.nlm.nih.gov/pubmed/21908659
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JB.05849-11
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