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Barcoded Primers Used in Multiplex Amplicon Pyrosequencing Bias Amplification
“Barcode-tagged” PCR primers used for multiplex amplicon sequencing generate a thus-far-overlooked amplification bias that produces variable terminal restriction fragment length polymorphism (T-RFLP) and pyrosequencing data from the same environmental DNA template. We propose a simple two-step PCR a...
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| Hauptverfasser: | , , , |
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| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
American Society for Microbiology
2011
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| Schlagworte: | |
| Online Zugang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3209180/ https://ncbi.nlm.nih.gov/pubmed/21890669 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.05220-11 |
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