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Error-correcting barcoded primers allow hundreds of samples to be pyrosequenced in multiplex
We have constructed error-correcting DNA barcodes that allow one run of a massively parallel pyrosequencer to process up to 1544 samples simultaneously. We have used these barcodes to process 16S ribosomal DNA sequences representing 286 microbial communities, correct 92% of sample assignment errors,...
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| Main Authors: | , , , , |
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| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
2008
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3439997/ https://ncbi.nlm.nih.gov/pubmed/18264105 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nmeth.1184 |
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