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Detection and quantification of adducts formed upon interaction of diamminedichloroplatinum (II) with DNA, by anion-exchange chromatography after enzymatic degradation.

A method has been developed to determine the adducts formed upon interaction of cis- and trans-diamminedichloroplatinum(II) (cis- and trans-DDP) with DNA. After 5 h at 50 degrees C in the dark, the amount of cis-DDP bound to salmon sperm DNA was larger than the amount of the trans-isomer. After enzy...

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Bibliografiske detaljer
Main Authors: Fichtinger-Schepman, A M, Lohman, P H, Reedijk, J
Format: Artigo
Sprog:Inglês
Udgivet: 1982
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC320876/
https://ncbi.nlm.nih.gov/pubmed/6216456
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