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Deletion loop mutagenesis: a novel method for the construction of point mutations using deletion mutants.

Deletion loop mutagenesis is a new, general method for site-directed mutagenesis that allows point mutations to the introduced within a sequence of DNA defined by a previously isolated deletion mutant. Wild type and deletion mutant DNA are cloned into a bacterial plasmid and each is cleaved with a d...

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Detalles Bibliográficos
Publicado en:Nucleic Acids Res
Principais autores: Kalderon, D, Oostra, B A, Ely, B K, Smith, A E
Formato: Artigo
Idioma:Inglês
Publicado: Oxford University Press 1982
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Acceso en liña:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC320862/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6292832/
https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/10.17.5161
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