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Deletion loop mutagenesis: a novel method for the construction of point mutations using deletion mutants.
Deletion loop mutagenesis is a new, general method for site-directed mutagenesis that allows point mutations to the introduced within a sequence of DNA defined by a previously isolated deletion mutant. Wild type and deletion mutant DNA are cloned into a bacterial plasmid and each is cleaved with a d...
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| Publicado no: | Nucleic Acids Res |
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| Main Authors: | , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Oxford University Press
1982
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC320862/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6292832/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/10.17.5161 |
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