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Deletion loop mutagenesis: a novel method for the construction of point mutations using deletion mutants.

Deletion loop mutagenesis is a new, general method for site-directed mutagenesis that allows point mutations to the introduced within a sequence of DNA defined by a previously isolated deletion mutant. Wild type and deletion mutant DNA are cloned into a bacterial plasmid and each is cleaved with a d...

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Autors principals: Kalderon, D, Oostra, B A, Ely, B K, Smith, A E
Format: Artigo
Idioma:Inglês
Publicat: 1982
Matèries:
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC320862/
https://ncbi.nlm.nih.gov/pubmed/6292832
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