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Stepwise Adaptations to Low Temperature as Revealed by Multiple Mutants of Psychrophilic α-Amylase from Antarctic Bacterium

The mutants Mut5 and Mut5CC from a psychrophilic α-amylase bear representative stabilizing interactions found in the heat-stable porcine pancreatic α-amylase but lacking in the cold-active enzyme from an Antarctic bacterium. From an evolutionary perspective, these mutants can be regarded as structur...

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מידע ביבליוגרפי
Main Authors: Cipolla, Alexandre, D'Amico, Salvino, Barumandzadeh, Roya, Matagne, André, Feller, Georges
פורמט: Artigo
שפה:Inglês
יצא לאור: American Society for Biochemistry and Molecular Biology 2011
נושאים:
גישה מקוונת:https://ncbi.nlm.nih.gov/pmc/articles/PMC3207396/
https://ncbi.nlm.nih.gov/pubmed/21900238
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1074/jbc.M111.274423
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