The Active Site Is the Least Stable Structure in the Unfolding Pathway of a Multidomain Cold-Adapted α-Amylase

The cold-active α-amylase from the Antarctic bacterium Pseudoalteromonas haloplanktis (AHA) is the largest known multidomain enzyme that displays reversible thermal unfolding (around 30°C) according to a two-state mechanism. Transverse urea gradient gel electrophoresis (TUG-GE) from 0 to 6.64 M was...

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Bibliographische Detailangaben
Hauptverfasser: Siddiqui, Khawar S., Feller, Georges, D'Amico, Salvino, Gerday, Charles, Giaquinto, Laura, Cavicchioli, Ricardo
Format: Artigo
Sprache:Inglês
Veröffentlicht: American Society for Microbiology 2005
Schlagworte:
Enzymes and Proteins
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC1196144/
https://ncbi.nlm.nih.gov/pubmed/16109961
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JB.187.17.6197-6205.2005
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