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Laser crosslinking of E. coli RNA polymerase and T7 DNA.
The first photochemical crosslinking of a protein to a nucleic acid using laser excitation is reported. A single, 120 mJ, 20 ns pulse at 248 nm crosslinks about 10% of bound E. coli RNA polymerase to T7 DNA under the conditions studied. The crosslinking yield depends on mercaptoethanol concentration...
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| Main Authors: | , , |
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| Format: | Artigo |
| Language: | Inglês |
| Published: |
1982
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| Online Access: | https://ncbi.nlm.nih.gov/pmc/articles/PMC320618/ https://ncbi.nlm.nih.gov/pubmed/7045809 |
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