Llwytho...
Laser crosslinking of E. coli RNA polymerase and T7 DNA.
The first photochemical crosslinking of a protein to a nucleic acid using laser excitation is reported. A single, 120 mJ, 20 ns pulse at 248 nm crosslinks about 10% of bound E. coli RNA polymerase to T7 DNA under the conditions studied. The crosslinking yield depends on mercaptoethanol concentration...
Wedi'i Gadw mewn:
| Prif Awduron: | , , |
|---|---|
| Fformat: | Artigo |
| Iaith: | Inglês |
| Cyhoeddwyd: |
1982
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| Mynediad Ar-lein: | https://ncbi.nlm.nih.gov/pmc/articles/PMC320618/ https://ncbi.nlm.nih.gov/pubmed/7045809 |
| Tagiau: |
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