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New procedure using a psoralen derivative for analysis of nucleosome associated DNA sequences in chromatin of living cells.

Sites for restriction endonuclease cleavage in double helical DNA are blocked from cleavage when the photoaffinity drug trimethylpsoralen is photobound at or near the site. In general, Hind III sites are about 15 fold more sensitive to inactivation than the other restriction sites which were tested,...

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Detalhes bibliográficos
Main Authors: Carlson, J O, Pfenninger, O, Sinden, R R, Lehman, J M, Pettijohn, D E
Formato: Artigo
Idioma:Inglês
Publicado em: 1982
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC320588/
https://ncbi.nlm.nih.gov/pubmed/6281729
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