Physiological Response to Membrane Protein Overexpression in E. coli

Overexpression represents a principal bottleneck in structural and functional studies of integral membrane proteins (IMPs). Although E. coli remains the leading organism for convenient and economical protein overexpression, many IMPs exhibit toxicity on induction in this host and give low yields of...

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Main Authors: Gubellini, Francesca, Verdon, Grégory, Karpowich, Nathan K., Luff, Jon D., Boël, Grégory, Gauthier, Nils, Handelman, Samuel K., Ades, Sarah E., Hunt, John F.
Formato: Artigo
Idioma:Inglês
Publicado: The American Society for Biochemistry and Molecular Biology 2011
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Research
Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC3205863/
https://ncbi.nlm.nih.gov/pubmed/21719796
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1074/mcp.M111.007930
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