Chicken histone H5: selection of a cDNA recombinant using an extended synthetic primer.

We describe the use of a synthetic primer to select a cDNA recombinant clone containing H5 coding sequences. The strategy used was as follows: 1. Prepare oligo(dT) cellulose-bound mRNA from chicken reticulocytes and select 11S-18S material from sucrose gradients. 2. Use this RNA fraction both to pre...

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Bibliographic Details
Main Authors: Krieg, P A, Robins, A J, Gait, M J, Titmas, R C, Wells, J R
Format: Artigo
Language:Inglês
Published: 1982
Subjects:
Research Article
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC320545/
https://ncbi.nlm.nih.gov/pubmed/6896089
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