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Chicken histone H5: selection of a cDNA recombinant using an extended synthetic primer.
We describe the use of a synthetic primer to select a cDNA recombinant clone containing H5 coding sequences. The strategy used was as follows: 1. Prepare oligo(dT) cellulose-bound mRNA from chicken reticulocytes and select 11S-18S material from sucrose gradients. 2. Use this RNA fraction both to pre...
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| Publicado en: | Nucleic Acids Res |
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| Autores principales: | , , , , |
| Formato: | Artigo |
| Lenguaje: | Inglês |
| Publicado: |
Oxford University Press
1982
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| Materias: | |
| Acceso en línea: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC320545/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6896089/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/10.5.1495 |
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