Gene shuttling: moving of cloned DNA into and out of eukaryotic cells.
Successful shuttling of cloned DNA in eukaryotic cells should allow isolation of expressed genes. We tested the utility of cosmids for moving DNA into and out of eukaryotic cells. The unique cleavage of DNA at the cos site by the terminase function of lambda was exploited to maintain the linkage bet...
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| Publicado no: | Nucleic Acids Res |
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| Principais autores: | , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Oxford University Press
1982
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC320522/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6280136/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/10.4.1243 |
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