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Molecular cloning of poliovirus cDNA and determination of the complete nucleotide sequence of the viral genome.
The complete 7410 nucleotide sequence of poliovirus type I genome was obtained from cloned cDNA. Double-stranded poliovirus cDNA was synthesized and inserted into the Pst I site of plasmid pBR322, and three clones were derived that together provided DNA copies of the entire poliovirus genome. Two of...
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| Udgivet i: | Proc Natl Acad Sci U S A |
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| Main Authors: | , |
| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
National Academy of Sciences
1981
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC320284/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6272282/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.78.8.4887 |
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