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Molecular cloning of poliovirus cDNA and determination of the complete nucleotide sequence of the viral genome.

The complete 7410 nucleotide sequence of poliovirus type I genome was obtained from cloned cDNA. Double-stranded poliovirus cDNA was synthesized and inserted into the Pst I site of plasmid pBR322, and three clones were derived that together provided DNA copies of the entire poliovirus genome. Two of...

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Veröffentlicht in:Proc Natl Acad Sci U S A
Hauptverfasser: Racaniello, V R, Baltimore, D
Format: Artigo
Sprache:Inglês
Veröffentlicht: National Academy of Sciences 1981
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC320284/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6272282/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.78.8.4887
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