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RF1 Knockout Allows Ribosomal Incorporation of Unnatural Amino Acids at Multiple Sites

Stop codons have been exploited for genetic incorporation of unnatural amino acids (Uaas) in live cells, but the efficiency is low possibly due to competition from release factors, limiting the power and scope of this technology. Here we show that the reportedly essential release factor 1 can be kno...

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Hlavní autoři: Johnson, David B.F., Xu, Jianfeng, Shen, Zhouxin, Takimoto, Jeffrey K., Schultz, Matthew D., Schmitz, Robert J., Ecker, Joseph R., Briggs, Steven P., Wang, Lei
Médium: Artigo
Jazyk:Inglês
Vydáno: 2011
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC3201715/
https://ncbi.nlm.nih.gov/pubmed/21926996
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nchembio.657
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