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Targeted mutation of the talpid3 gene in zebrafish reveals its conserved requirement for ciliogenesis and Hedgehog signalling across the vertebrates

Using zinc-finger nuclease-mediated mutagenesis, we have generated mutant alleles of the zebrafish orthologue of the chicken talpid3 (ta3) gene, which encodes a centrosomal protein that is essential for ciliogenesis. Animals homozygous for these mutant alleles complete embryogenesis normally, but ma...

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Detalhes bibliográficos
Main Authors: Ben, Jin, Elworthy, Stone, Ng, Ashley Shu Mei, van Eeden, Freek, Ingham, Philip W.
Formato: Artigo
Idioma:Inglês
Publicado em: Company of Biologists 2011
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3201664/
https://ncbi.nlm.nih.gov/pubmed/22028029
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1242/dev.070862
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