Oligonucleotide directed mutagenesis of Escherichia coli 5S ribosomal RNA: construction of mutant and structural analysis.

The ribosomal 5S RNA gene from the rrnB operon of E. coli was mutagenised in vitro using a synthetic oligonucleotide hybridised to M13 ssDNA containing that gene. The oligonucleotide corresponded to the 5S RNA sequence positions 34 to 51 and changed the guanosine at position 41 to a cytidine. The DN...

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Detalhes bibliográficos

Publicado no:	Nucleic Acids Res
Principais autores:	Göringer, H U, Wagner, R, Jacob, W F, Dahlberg, A E, Zwieb, C
Formato:	Artigo
Idioma:	Inglês
Publicado em:	Oxford University Press 1984
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC320134/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6091046/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/12.18.6935
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