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Combinatorial Library Screening for Developing an Improved–Split- Firefly Luciferase Fragment-Assisted Complementation System for Studying Protein-Protein Interactions

Split reporter based bioluminescence imaging is a useful strategy for studying protein-protein as well as other intracellular interactions. We have used a combinatorial strategy to identify a novel split site for firefly luciferase with improved characteristics over previously published split sites....

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Détails bibliographiques
Auteurs principaux: Paulmurugan, Ramasamy, Gambhir, Sanjiv S.
Format: Artigo
Langue:Inglês
Publié: 2007
Sujets:
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC3198827/
https://ncbi.nlm.nih.gov/pubmed/17295448
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/ac062053q
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