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Decontamination of MDA Reagents for Single Cell Whole Genome Amplification

Single cell genomics is a powerful and increasingly popular tool for studying the genetic make-up of uncultured microbes. A key challenge for successful single cell sequencing and analysis is the removal of exogenous DNA from whole genome amplification reagents. We found that UV irradiation of the m...

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Hlavní autoři: Woyke, Tanja, Sczyrba, Alexander, Lee, Janey, Rinke, Christian, Tighe, Damon, Clingenpeel, Scott, Malmstrom, Rex, Stepanauskas, Ramunas, Cheng, Jan-Fang
Médium: Artigo
Jazyk:Inglês
Vydáno: Public Library of Science 2011
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On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC3197606/
https://ncbi.nlm.nih.gov/pubmed/22028825
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0026161
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