Quantification of Proteins Using Peptide Immunoaffinity Enrichment Coupled with Mass Spectrometry

There is a great need for quantitative assays in measuring proteins. Traditional sandwich immunoassays, largely considered the gold standard in quantitation, are associated with a high cost, long lead time, and are fraught with drawbacks (e.g. heterophilic antibodies, autoantibody interference, ...

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Detalhes bibliográficos
Main Authors: Zhao, Lei, Whiteaker, Jeffrey R., Pope, Matthew E., Kuhn, Eric, Jackson, Angela, Anderson, N. Leigh, Pearson, Terry W., Carr, Steven A., Paulovich, Amanda G.
Formato: Artigo
Idioma:Inglês
Publicado em: MyJove Corporation 2011
Assuntos:
Molecular Biology
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3197439/
https://ncbi.nlm.nih.gov/pubmed/21841765
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/2812
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