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Use of periplasmic target protein capture for phage display engineering of tight-binding protein–protein interactions

Phage display is a powerful tool to study and engineer protein and peptide interactions. It is not without its limitations, however, such as the requirement for target protein purification and immobilization in a correctly folded state. A protein capture method is described here that allows enrichme...

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Hlavní autoři: Fryszczyn, Bartlomiej G., Brown, Nicholas G., Huang, Wanzhi, Balderas, Miriam A., Palzkill, Timothy
Médium: Artigo
Jazyk:Inglês
Vydáno: Oxford University Press 2011
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC3196869/
https://ncbi.nlm.nih.gov/pubmed/21900304
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/protein/gzr043
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