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Use of periplasmic target protein capture for phage display engineering of tight-binding protein–protein interactions
Phage display is a powerful tool to study and engineer protein and peptide interactions. It is not without its limitations, however, such as the requirement for target protein purification and immobilization in a correctly folded state. A protein capture method is described here that allows enrichme...
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| Main Authors: | , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Oxford University Press
2011
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3196869/ https://ncbi.nlm.nih.gov/pubmed/21900304 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/protein/gzr043 |
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