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Separation and characterization of inner and outer envelope membranes of pea chloroplasts

A procedure for purifying the chloroplast envelope subfractionates it into two membrane fractions of comparable quantities. This procedure differs from previous ones in that the chloroplasts are ruptured by freezing and thawing in hypertonic medium rather than by osmotic shock. The two membrane frac...

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Détails bibliographiques
Publié dans:Proc Natl Acad Sci U S A
Auteurs principaux: Cline, Kenneth, Andrews, Jaen, Mersey, Brent, Newcomb, Eldon H., Keegstra, Kenneth
Format: Artigo
Langue:Inglês
Publié: National Academy of Sciences 1981
Sujets:
Accès en ligne:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC319617/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/16593034/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.78.6.3595
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