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Separation and characterization of inner and outer envelope membranes of pea chloroplasts

A procedure for purifying the chloroplast envelope subfractionates it into two membrane fractions of comparable quantities. This procedure differs from previous ones in that the chloroplasts are ruptured by freezing and thawing in hypertonic medium rather than by osmotic shock. The two membrane frac...

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Detalhes bibliográficos
Main Authors: Cline, Kenneth, Andrews, Jaen, Mersey, Brent, Newcomb, Eldon H., Keegstra, Kenneth
Formato: Artigo
Idioma:Inglês
Publicado em: 1981
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC319617/
https://ncbi.nlm.nih.gov/pubmed/16593034
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