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Separation and characterization of inner and outer envelope membranes of pea chloroplasts

A procedure for purifying the chloroplast envelope subfractionates it into two membrane fractions of comparable quantities. This procedure differs from previous ones in that the chloroplasts are ruptured by freezing and thawing in hypertonic medium rather than by osmotic shock. The two membrane frac...

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Bibliographische Detailangaben
Veröffentlicht in:Proc Natl Acad Sci U S A
Hauptverfasser: Cline, Kenneth, Andrews, Jaen, Mersey, Brent, Newcomb, Eldon H., Keegstra, Kenneth
Format: Artigo
Sprache:Inglês
Veröffentlicht: National Academy of Sciences 1981
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC319617/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/16593034/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.78.6.3595
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