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In vitro Reconstitution of the Active T. castaneum Telomerase

Efforts to isolate the catalytic subunit of telomerase, TERT, in sufficient quantities for structural studies, have been met with limited success for more than a decade. Here, we present methods for the isolation of the recombinant Tribolium castaneum TERT (TcTERT) and the reconstitution of the acti...

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Detalhes bibliográficos
Main Authors: Schuller, Anthony P., Harkisheimer, Michael J., Skordalakes, Emmanuel
Formato: Artigo
Idioma:Inglês
Publicado em: MyJove Corporation 2011
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3196162/
https://ncbi.nlm.nih.gov/pubmed/21775967
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/2799
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