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An overview of enzymatic reagents for the removal of affinity tags

Although they are often exploited to facilitate the expression and purification of recombinant proteins, every affinity tag, whether large or small, has the potential to interfere with the structure and function of its fusion partner. For this reason, reliable methods for removing affinity tags are...

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Dettagli Bibliografici
Autore principale: Waugh, David S.
Natura: Artigo
Lingua:Inglês
Pubblicazione: Academic Press 2011
Soggetti:
Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC3195948/
https://ncbi.nlm.nih.gov/pubmed/21871965
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.pep.2011.08.005
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