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The substrate specificity of Metarhizium anisopliae and Bos taurus carboxypeptidases A: Insights into their use as tools for the removal of affinity tags

Carboxypeptidases may serve as tools for removal for C-terminal affinity tags. In the present study, we describe the expression and purification of an A-type carboxypeptidase from the fungal pathogen Metarhizium anisopliae (MeCPA) that has been genetically engineered to facilitate the removal of pol...

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Detalhes bibliográficos
Main Authors: Austin, Brian P., Tözsér, József, Bagossi, Péter, Tropea, Joseph E., Waugh, David S.
Formato: Artigo
Idioma:Inglês
Publicado em: 2010
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3412371/
https://ncbi.nlm.nih.gov/pubmed/21073956
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.pep.2010.11.005
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