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An enzymatic colorimetric assay for glucose-6-phosphate

A specific colorimetric assay for the determination of glucose-6-phosphate (G6P) was developed. This assay is based on the oxidation of G6P in the presence of glucose-6-phosphate dehydrogenase (G6PD) and NADP(+); the NADPH thereby generated reduces the tetrazolium salt WST-1 (2-(4-indophenyl)-3-(4-n...

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Detalhes bibliográficos
Main Authors: Zhu, Aiping, Romero, Roberto, Petty, Howard R.
Formato: Artigo
Idioma:Inglês
Publicado em: 2011
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3195850/
https://ncbi.nlm.nih.gov/pubmed/21925475
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ab.2011.08.037
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