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A sensitive fluorimetric assay for pyruvate

A sensitive and specific fluorimetric assay for the determination of pyruvate is reported. This assay is based on the oxidation of pyruvate in the presence of pyruvate oxidase. Hydrogen peroxide generated by pyruvate oxidase reacts with non-fluorescent amplex red at a 1:1 stoichiometry to form the f...

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Detalhes bibliográficos
Main Authors: Zhu, Aiping, Romero, Roberto, Petty, Howard R.
Formato: Artigo
Idioma:Inglês
Publicado em: 2009
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2790020/
https://ncbi.nlm.nih.gov/pubmed/19748474
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ab.2009.09.017
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