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Detecting Protein-Ligand Binding on Supported Bilayers by Local pH Modulation
Herein, we describe a highly sensitive technique for detecting protein-ligand binding at the liquid/solid interface. The method is based upon modulation of the interfacial pH when the protein binds. This change is detected by ortho-Texas Red DHPE, which is doped into supported phospholipid bilayers...
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| Main Authors: | , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2009
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3195364/ https://ncbi.nlm.nih.gov/pubmed/19125648 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/ja804542p |
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