DNA breakage and closure by rat liver type 1 topoisomerase: separation of the half-reactions by using a single-stranded DNA substrate.

Circular single strands of bacteriophage phi X174 DNA are broken by rat liver DNA nicking-closing enzyme (type 1 topoisomerase) in low salt (50 mM KCl) at 37 degrees C, generating linear strands containing covalently bound enzyme [Been, M. D. & Champoux, J. J. (1980) Nucleic Acids Res. 8, 6129-6142]...

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Detalhes bibliográficos

Publicado no:	Proc Natl Acad Sci U S A
Principais autores:	Been, M D, Champoux, J J
Formato:	Artigo
Idioma:	Inglês
Publicado em:	National Academy of Sciences 1981
Assuntos:	Research Article
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC319463/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6265921/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.78.5.2883
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