Molecular cloning and characterization of winter flounder antifreeze cDNA.
Double-stranded cDNA was synthesized from partially purified winter flounder antifreeze mRNA and inserted into the endonuclease Pst I site of plasmid pBR322 by the poly(dG).poly(dC) homopolymer extension technique. The recombinant plasmids wee used to transform Escherichia coli. Clones containing an...
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| Udgivet i: | Proc Natl Acad Sci U S A |
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| Principais autores: | , |
| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
National Academy of Sciences
1981
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC319450/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6265915/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.78.5.2825 |
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