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Molecular cloning and characterization of winter flounder antifreeze cDNA.

Double-stranded cDNA was synthesized from partially purified winter flounder antifreeze mRNA and inserted into the endonuclease Pst I site of plasmid pBR322 by the poly(dG).poly(dC) homopolymer extension technique. The recombinant plasmids wee used to transform Escherichia coli. Clones containing an...

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Bibliografske podrobnosti
Main Authors: Lin, Y, Gross, J K
Format: Artigo
Jezik:Inglês
Izdano: 1981
Teme:
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC319450/
https://ncbi.nlm.nih.gov/pubmed/6265915
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