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Yeast genes fused to beta-galactosidase in Escherichia coli can be expressed normally in yeast.
A plasmid was constructed that allows the selection in vivo of gene fusions between the Escherichia coli beta-galactosidase gene and the yeast (Saccharomyces cerevisiae) URA3 gene. A large yeast DNA fragment containing the URA3 gene was placed upstream of an amino-terminally deleted version of the l...
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| 發表在: | Proc Natl Acad Sci U S A |
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| Main Authors: | , , |
| 格式: | Artigo |
| 語言: | Inglês |
| 出版: |
National Academy of Sciences
1981
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| 主題: | |
| 在線閱讀: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC319366/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6787605/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.78.4.2460 |
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