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Genetic complementation of the Saccharomyces cerevisiae leu2 gene by the Escherichia coli leuB gene.
The leucine operon of Escherichia coli was cloned on a plasmid possessing both E. coli and Saccharomyces cerevisiae replication origins. This plasmid, pEH25, transformed leuA, leuB, and leuD auxotrophs of E. coli to prototrophy; it also transformed leu2 auxotrophs of S. cerevisiae to prototrophy. be...
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| Udgivet i: | Mol Cell Biol |
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| Main Authors: | , , |
| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
Taylor & Francis
1981
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC369367/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9279396/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/mcb.1.9.836 |
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