Subtissue-Specific Evaluation of Promoter Efficiency by Quantitative Fluorometric Assay in Laser Microdissected Tissues of Rapeseed([W])

β-Glucuronidase (GUS) is a useful reporter for the evaluation of promoter characteristics in transgenic plants. Here, we introduce an original technique to quantify the strength of promoters at subtissue resolution of cell clusters. The method combines cryotomy, laser microdissection, and improved f...

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Bibliografiske detaljer
Main Authors: Jasik, Jan, Schiebold, Silke, Rolletschek, Hardy, Denolf, Peter, Van Adenhove, Katrien, Altmann, Thomas, Borisjuk, Ljudmilla
Format: Artigo
Sprog:Inglês
Udgivet: American Society of Plant Biologists 2011
Fag:
Breakthrough Technologies
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3192573/
https://ncbi.nlm.nih.gov/pubmed/21825109
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1104/pp.111.180760
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