Subtissue-Specific Evaluation of Promoter Efficiency by Quantitative Fluorometric Assay in Laser Microdissected Tissues of Rapeseed([W])

β-Glucuronidase (GUS) is a useful reporter for the evaluation of promoter characteristics in transgenic plants. Here, we introduce an original technique to quantify the strength of promoters at subtissue resolution of cell clusters. The method combines cryotomy, laser microdissection, and improved f...

Täydet tiedot

Tallennettuna:
Bibliografiset tiedot
Päätekijät: Jasik, Jan, Schiebold, Silke, Rolletschek, Hardy, Denolf, Peter, Van Adenhove, Katrien, Altmann, Thomas, Borisjuk, Ljudmilla
Aineistotyyppi: Artigo
Kieli:Inglês
Julkaistu: American Society of Plant Biologists 2011
Aiheet:
Breakthrough Technologies
Linkit:https://ncbi.nlm.nih.gov/pmc/articles/PMC3192573/
https://ncbi.nlm.nih.gov/pubmed/21825109
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1104/pp.111.180760
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