Excision of transposon Tn5 is dependent on the inverted repeats but not on the transposase function of Tn5.
The excision of Tn5 from sites of insertion in the Escherichia coli genome was studied by examining the reversion of lac::Tn5 insertion mutations to lac+. We find that: (i) the frequency of excision depends on the site of Tn5 insertion, (ii) excision occurs efficiently in recA- cells, (iii) excision...
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| Udgivet i: | Proc Natl Acad Sci U S A |
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| Principais autores: | , |
| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
National Academy of Sciences
1981
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC319073/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6264444/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.78.1.459 |
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