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Chromosomes in living Escherichia coli cells are segregated into domains of supercoiling.

Torsional tension in the DNA double helix can be detected in living cells of Escherichia coli from measurements of the rate of trimethylpsoralen photobinding to the intracellular DNA. Here we show that this tension is relaxed in vivo when single-strand DNA breaks are introduced by gamma-irradiation...

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Détails bibliographiques
Publié dans:Proc Natl Acad Sci U S A
Auteurs principaux: Sinden, R R, Pettijohn, D E
Format: Artigo
Langue:Inglês
Publié: National Academy of Sciences 1981
Sujets:
Accès en ligne:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC319024/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6165987/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.78.1.224
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