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High-efficiency Gene Targeting in Schizosaccharomyces pombe Using a Modular, PCR-based Approach with Long Tracts of Flanking Homology
Bähler et al. (1998) recently described a PCR-based system for the deletion, tagging and overexpression of endogenous genes in the fission yeast Schizosaccharomyces pombe. A small set of PCR primers can be used to generate gene-targeting substrates from each of several modules that differ in the sel...
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| Main Authors: | , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado: |
1999
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| Assuntos: | |
| Acceso en liña: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3190138/ https://ncbi.nlm.nih.gov/pubmed/10509024 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/(SICI)1097-0061(19990930)15:13<1419::AID-YEA466>3.0.CO;2-Q |
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