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Weak Seed-Pairing Stability and High Target-Site Abundance Decrease the Proficiency of lsy-6 and Other miRNAs

Most metazoan microRNAs (miRNAs) target many genes for repression, but the nematode lsy-6 miRNA is much less proficient. Here, we show that the low proficiency of lsy-6 can be recapitulated in HeLa cells and that miR-23 (a mammalian miRNA) also has low proficiency in these cells. Reporter results an...

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Autors principals: Garcia, David M., Baek, Daehyun, Shin, Chanseok, Bell, George W., Grimson, Andrew, Bartel, David P.
Format: Artigo
Idioma:Inglês
Publicat: 2011
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC3190056/
https://ncbi.nlm.nih.gov/pubmed/21909094
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nsmb.2115
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