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Site-directed mutagenesis of the Escherichia coli chromosome near oriC: identification and characterization of asnC, a regulatory element in E. coli asparagine metabolism.

We developed a new method for the specific mutagenization of the E. coli chromosome. This method takes advantage of the fact that a pBR322 plasmid containing chromosomal sequences is mobilizable during an Hfr-mediated conjugational transfer, due to an homologous recombination between the E. coli Hfr...

תיאור מלא

שמור ב:
מידע ביבליוגרפי
Main Authors: de Wind, N, de Jong, M, Meijer, M, Stuitje, A R
פורמט: Artigo
שפה:Inglês
יצא לאור: 1985
גישה מקוונת:https://ncbi.nlm.nih.gov/pmc/articles/PMC318952/
https://ncbi.nlm.nih.gov/pubmed/3909107
תגים: הוספת תג
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