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A new experimental approach for studying the association between RNA polymerase and the tet promoter of pBR322.
In order to follow the kinetics of the initiation of transcription by the E. coli RNA polymerase, we have used the procedure of abortive initiation as described by Mc Clure (1980) (7). In place of radioactive labeling we have taken advantage of a fluorescent probe (UTP gamma ANS) to obtain fast and...
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| Main Authors: | , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
1984
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| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC318609/ https://ncbi.nlm.nih.gov/pubmed/6366739 |
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