Effect of superhelicity on the transcription from the tet promoter of pBR322. Abortive initiation and unwinding experiments.
Supercoiling of DNA is now known to have considerable effects on transcription in bacteria. By abortive initiation reaction (6) we have determined the binding constant KB and the forward rate of isomerization k2 as a function of temperature, pH and buffer for the tet promoter in a supercoiled plasmi...
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| 發表在: | Nucleic Acids Res |
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| Principais autores: | , , , |
| 格式: | Artigo |
| 語言: | Inglês |
| 出版: |
Oxford University Press
1984
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| 在線閱讀: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC320197/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6387626/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/12.20.7741 |
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